Phospho-ELk1 (Ser389) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5335r-100uL |
| Product Name | Phospho-ELk1 (Ser389) Polyclonal Antibody |
| Applications |
WB
ELISA
FCM
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ELk1 around the phosphorylation site of Ser389 [PR(p-S)PA] |
| Modification Site | Ser389 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2002 |
| Subcellular location | Nucleus |
| Synonyms | Elk-1Ser389; ELK1 phospho S389; p-ELK1 phospho S389; ELK 1; ELK1 member of ETS oncogene family; ELK1 protein; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; Oncogene Elk1; ETS domain-containing protein Elk-1 isoform a; ETS domain-containing protein Elk-1; ETS-like gene 1; tyrosine kinase ELK1 oncogene; ELK1_HUMAN. |
| Background | This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1ug/Test |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-ELk1 (Ser389) Polyclonal Antibody (bs-5335R) validated for, and at what dilution?
bs-5335R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1ug/Test); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-ELk1 (Ser389) Polyclonal Antibody (bs-5335R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Rabbit, GuineaPig.
How should bs-5335R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-ELk1 (Ser389) Polyclonal Antibody (bs-5335R)?
KLH conjugated synthetic phosphopeptide derived from human ELk1 around the phosphorylation site of Ser389 [PR(p-S)PA]