phospho-EP300 (Ser1834) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5339R-FITC |
| Product Name | phospho-EP300 (Ser1834) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken, Guinea Pig |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834 |
| Modification Site | Ser1834 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2033 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CREBBP/EP300 inhibitory protein 1; Cyclic AMP responsive enhancer binding protein; E1A associated protein p300; E1A binding protein p300; EC 2.3.1.48; EP300; EP300: E1A binding protein p300; RB and P300 binding protein EID 1; Histone acetyltransferase p300; p300 HAT; Retinoblastoma protein associated protein. |
| Background | KAT3B/p300 is a transcriptional adapter protein (300 kDa)which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP)complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A/CBP(for CREB-binding protein). KAT3B and KAT3A are homologous to each other. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-EP300 (Ser1834) Polyclonal Antibody, FITC Conjugated (bs-5339R-FITC) validated for, and at what dilution?
bs-5339R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-EP300 (Ser1834) Polyclonal Antibody, FITC Conjugated (bs-5339R-FITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, Guinea Pig.
How should bs-5339R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-EP300 (Ser1834) Polyclonal Antibody, FITC Conjugated (bs-5339R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834
What are the excitation and emission wavelengths of the FITC conjugate (bs-5339R-FITC)?
FITC on bs-5339R-FITC: excitation / emission 494nm/518nm.