Phospho-ERBB3(Tyr1328) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5340R-BF405-100uL |
| Product Name | Phospho-ERBB3(Tyr1328) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HER3 around the phosphorylation site of Tyr1328 [PD(p-Y)WH] |
| Modification Site | Tyr1328 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2065 |
| Swiss Prot | P21860 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; ERBB3 |
| Background | ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. Isoform 2 lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized. Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2); also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-ERBB3(Tyr1328) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5340R-BF405) validated for, and at what dilution?
bs-5340R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-ERBB3(Tyr1328) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5340R-BF405) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Pig, Horse, Chicken, Rabbit.
How should bs-5340R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-ERBB3(Tyr1328) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5340R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human HER3 around the phosphorylation site of Tyr1328 [PD(p-Y)WH]
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-5340R-BF405)?
AbBy Fluor-405 on bs-5340R-BF405: excitation / emission 401nm/421nm.