phospho-ECT2 (Thr373) Polyclonal Antibody, HRP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5344R-HRP |
| Product Name | phospho-ECT2 (Thr373) Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Dog, Cow, Sheep |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ECT2 around the phosphorylation site of Thr373 |
| Modification Site | Thr373 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1894 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ECT2 phospho T373; ECT2 phospho Thr373; p-ECT2 Thr373; ECT 2; ECT2; ECT-2; ECT2 protein; Epithelial cell transforming sequence 2; Epithelial cell transforming sequence 2 oncogene; Epithelial cell transforming sequence 2 oncogene protein; FLJ10461; MGC138291; Protein ECT2; ECT2_HUMAN; Epithelial cell-transforming sequence 2 oncogene. |
| Background | The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-ECT2 (Thr373) Polyclonal Antibody, HRP Conjugated (bs-5344R-HRP) validated for, and at what dilution?
bs-5344R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5344R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ECT2 (Thr373) Polyclonal Antibody, HRP Conjugated (bs-5344R-HRP)?
KLH conjugated synthetic phosphopeptide derived from human ECT2 around the phosphorylation site of Thr373