phospho-FXYD1 (Ser83) Polyclonal Antibody, Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5348R-Cy7 |
| Product Name | phospho-FXYD1 (Ser83) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FXYD1 around the phosphorylation site of Ser83 |
| Modification Site | Ser83 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5348 |
| Subcellular location | Cell membrane |
| Synonyms | FXYD1 phospho S83; FXYD1 phospho Ser83; p-FXYD1 phospho S83; FXYD domain containing ion transport regulator 1; Phospholemman; PLM; fxyd1; PLM_HUMAN; FXYD domain-containing ion transport regulator 1. |
| Background | PLM (FXYD1)is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-FXYD1 (Ser83) Polyclonal Antibody, Cy7 Conjugated (bs-5348R-Cy7) validated for, and at what dilution?
bs-5348R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5348R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-FXYD1 (Ser83) Polyclonal Antibody, Cy7 Conjugated (bs-5348R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human FXYD1 around the phosphorylation site of Ser83
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5348R-Cy7)?
Cy7 on bs-5348R-Cy7: excitation / emission 743nm/767nm.