phospho-Gria2 (Ser880) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5359R-BF405-100uL |
| Product Name | phospho-Gria2 (Ser880) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Gria2 around the phosphorylation site of Ser880 |
| Modification Site | Ser880 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2891 |
| Subcellular location | Cell membrane |
| Synonyms | Glutamate receptor 2 PrecursorSer880; Gria2; Glur-2; GluR-B; Glur2; GluR-2; GluR-K2; Glutamate receptor 2; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA receptor subunit ; AMPA-selective glutamate receptor 2. |
| Background | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing(CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Gria2 (Ser880) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5359R-BF405) validated for, and at what dilution?
bs-5359R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Gria2 (Ser880) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5359R-BF405) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Chicken.
How should bs-5359R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Gria2 (Ser880) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5359R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human Gria2 around the phosphorylation site of Ser880
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-5359R-BF405)?
AbBy Fluor-405 on bs-5359R-BF405: excitation / emission 401nm/421nm.