phospho-Gria2 (Ser880) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5359R-Biotin |
| Product Name | phospho-Gria2 (Ser880) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Gria2 around the phosphorylation site of Ser880 |
| Modification Site | Ser880 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 2891 |
| Subcellular location | Cell membrane |
| Synonyms | Glutamate receptor 2 PrecursorSer880; Gria2; Glur-2; GluR-B; Glur2; GluR-2; GluR-K2; Glutamate receptor 2; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA receptor subunit ; AMPA-selective glutamate receptor 2. |
| Background | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing(CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-Gria2 (Ser880) Polyclonal Antibody, Biotin Conjugated (bs-5359R-Biotin) validated for, and at what dilution?
bs-5359R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Gria2 (Ser880) Polyclonal Antibody, Biotin Conjugated (bs-5359R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Chicken.
How should bs-5359R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Gria2 (Ser880) Polyclonal Antibody, Biotin Conjugated (bs-5359R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human Gria2 around the phosphorylation site of Ser880