phospho-GIT1 (Tyr510) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5374R-Cy5.5-100uL |
| Product Name | phospho-GIT1 (Tyr510) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr510 |
| Modification Site | Tyr510 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 28964 |
| Swiss Prot | Q9Y2X7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; GIT1 |
| Background | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-GIT1 (Tyr510) Polyclonal Antibody, Cy5.5 Conjugated (BS-5374R-CY5-5) validated for, and at what dilution?
BS-5374R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GIT1 (Tyr510) Polyclonal Antibody, Cy5.5 Conjugated (BS-5374R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Pig, Chicken.
How should BS-5374R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GIT1 (Tyr510) Polyclonal Antibody, Cy5.5 Conjugated (BS-5374R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr510
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5374R-CY5-5)?
Cy5.5 on BS-5374R-CY5-5: excitation / emission 675nm/694nm.