phospho-HMG14 (Ser20+Ser24) Polyclonal Antibody, Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5384R-Cy7 |
| Product Name | phospho-HMG14 (Ser20+Ser24) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HMGN1 around the phosphorylation site of Ser20+Ser24 |
| Modification Site | Ser21+Ser25 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3150 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HMG14 phospho S21 + S25; High mobility group nonhistone chromosomal protein 14; High mobility group nucleosome binding 1; High mobility group nucleosome binding domain containing protein 1; High mobility group protein 14; High-mobility group nucleosome binding domain 1; HMG14; HMGN 1; HMGN1; MGC104230; MGC117425; Nonhistone chromosomal protein HMG-14; Nonhistone chromosomal protein HMG14; FLJ27265; FLJ31471. |
| Background | Chromosomal protein HMG14 binds to the inner side of the nucleosomal DNA, thus altering the interaction between the DNA and the histone octamer and is involved in the process that maintains transcribable genes in an appropriate chromatin conformation. HMG14 has been implicated as a contributing factor in the etiology of Down syndrome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-HMG14 (Ser20+Ser24) Polyclonal Antibody, Cy7 Conjugated (bs-5384R-Cy7) validated for, and at what dilution?
bs-5384R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5384R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HMG14 (Ser20+Ser24) Polyclonal Antibody, Cy7 Conjugated (bs-5384R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human HMGN1 around the phosphorylation site of Ser20+Ser24
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5384R-Cy7)?
Cy7 on bs-5384R-Cy7: excitation / emission 743nm/767nm.