phospho-HDAC2 (Ser394) Polyclonal Antibody

$Antigen: bs-5389P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-5389P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse
Chicken

Overview
Catalog #	bs-5389R
Product Name	phospho-HDAC2 (Ser394) Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of Ser394
Modification Site	Ser394
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3066
Subcellular location	Nucleus
Synonyms	HDAC2 Ser394; HDAC2 phospho S394; HDAC2 phospho Ser394; p-HDAC2 Ser394; histone deacetylase 2; D10Wsu179e; EC 3.5.1.98; HD2; HDAC 2; Hdac2; histone deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1 associated factor 1; YY1 transcription factor binding protein; Yy1bp; HDAC2_HUMAN.
Background	This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010].
Application Dilution
WB	ELISA1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF	IHC-P1:50-200
Flow-Cyt	IFIHC-F1:50-200
ICC/IF	IFICC1:50-200