phospho-HDAC2 (Ser394) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5389R-FITC |
| Product Name | phospho-HDAC2 (Ser394) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of Ser394 |
| Modification Site | Ser394 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3066 |
| Subcellular location | Nucleus |
| Synonyms | HDAC2 Ser394; HDAC2 phospho S394; HDAC2 phospho Ser394; p-HDAC2 Ser394; histone deacetylase 2; D10Wsu179e; EC 3.5.1.98; HD2; HDAC 2; Hdac2; histone deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1 associated factor 1; YY1 transcription factor binding protein; Yy1bp; HDAC2_HUMAN. |
| Background | This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]. |
| Application Dilution | |
| WB | IFIHC-P1:50-200 |
| IF | IHC-F1:50-200 |
Frequently asked questions
Which applications is phospho-HDAC2 (Ser394) Polyclonal Antibody, FITC Conjugated (bs-5389R-FITC) validated for, and at what dilution?
bs-5389R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HDAC2 (Ser394) Polyclonal Antibody, FITC Conjugated (bs-5389R-FITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken.
How should bs-5389R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HDAC2 (Ser394) Polyclonal Antibody, FITC Conjugated (bs-5389R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of Ser394
What are the excitation and emission wavelengths of the FITC conjugate (bs-5389R-FITC)?
FITC on bs-5389R-FITC: excitation / emission 494nm/518nm.