Seladin 1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5390R-Cy3-100uL |
| Product Name | Seladin 1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Seladin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1718 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Seladin1; Seladin-1; DHCR24; 24 dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; DCE; Desmosterol to cholesterol enzyme; Diminuto dwarf1 homolog; SELADIN1; Selective AD indicator 1; Selective Alzheimer's Disease Indicator 1. |
| Background | Seladin 1 is highly expressed in brain, adrenal tissues and adrenal tumours, where this enzyme catalyzes the reduction of the delta-24 double bond of sterol intermediates such as desmosterol, during cholesterol biosynthesis. In addition, Seladin 1 has an anti-apoptotic function by reducing caspase 3 activity during oxidative stress-induced apoptosis and protecting cells against amyloid-beta peptide-induced apoptosis. Several studies have identified a reduction in expression levels of this enzyme in brain regions affected by Alzheimer's disease (hippocampus and the subventricular zone). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Seladin 1 Polyclonal Antibody, Cy3 Conjugated (bs-5390R-Cy3) validated for, and at what dilution?
bs-5390R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Seladin 1 Polyclonal Antibody, Cy3 Conjugated (bs-5390R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Rabbit.
How should bs-5390R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Seladin 1 Polyclonal Antibody, Cy3 Conjugated (bs-5390R-Cy3)?
KLH conjugated synthetic peptide derived from human Seladin 1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5390R-Cy3)?
Cy3 on bs-5390R-Cy3: excitation / emission 512,550nm/570,615nm.