phospho-HOMER3 (Thr36) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5392R-RBITC-100uL |
| Product Name | phospho-HOMER3 (Thr36) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HOMER3 around the phosphorylation site of Thr36 [AL(p-T)VS] |
| Modification Site | Thr36 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9454 |
| Swiss Prot | Q9NSC5 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | VESL3; HOMER-3; Homer protein homolog 3; HOMER3 |
| Background | Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-HOMER3 (Thr36) Polyclonal Antibody, RBITC Conjugated (bs-5392R-RBITC) validated for, and at what dilution?
bs-5392R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HOMER3 (Thr36) Polyclonal Antibody, RBITC Conjugated (bs-5392R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Chicken.
How should bs-5392R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HOMER3 (Thr36) Polyclonal Antibody, RBITC Conjugated (bs-5392R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human HOMER3 around the phosphorylation site of Thr36 [AL(p-T)VS]
What are the excitation and emission wavelengths of the RBITC conjugate (bs-5392R-RBITC)?
RBITC on bs-5392R-RBITC: excitation / emission 540nm/625nm.