phospho-IL7R (Tyr449) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5395R-Cy3 |
| Product Name | phospho-IL7R (Tyr449) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IL-7Ra around the phosphorylation site of Tyr449 |
| Modification Site | Tyr449 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3575 |
| Swiss Prot | P16871 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | ILRA; CD127; IL7RA; CDW127; IL-7R-alpha; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7RA; IL7R |
| Background | Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IL7R (Tyr449) Polyclonal Antibody, Cy3 Conjugated (bs-5395R-Cy3) validated for, and at what dilution?
bs-5395R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IL7R (Tyr449) Polyclonal Antibody, Cy3 Conjugated (bs-5395R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken.
How should bs-5395R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IL7R (Tyr449) Polyclonal Antibody, Cy3 Conjugated (bs-5395R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human IL-7Ra around the phosphorylation site of Tyr449
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5395R-Cy3)?
Cy3 on bs-5395R-Cy3: excitation / emission 512,550nm/570,615nm.