phospho-IRS1 (Ser312) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5396R-Cy5.5-100uL |
| Product Name | phospho-IRS1 (Ser312) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser312 [AT(p-S)PA] |
| Modification Site | Ser312 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3667 |
| Subcellular location | Cell membrane |
| Synonyms | IRS-1 Ser312; p-IRS1; IRS1Ser789; HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS-1; IRS 1; IRS1; OTTHUMP00000164234; IRS1_HUMAN. |
| Background | Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IRS1 (Ser312) Polyclonal Antibody, Cy5.5 Conjugated (BS-5396R-CY5-5) validated for, and at what dilution?
BS-5396R-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IRS1 (Ser312) Polyclonal Antibody, Cy5.5 Conjugated (BS-5396R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should BS-5396R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IRS1 (Ser312) Polyclonal Antibody, Cy5.5 Conjugated (BS-5396R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser312 [AT(p-S)PA]
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5396R-CY5-5)?
Cy5.5 on BS-5396R-CY5-5: excitation / emission 675nm/694nm.