phospho-IRS-2(Ser731) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5397R-BF405 |
| Product Name | phospho-IRS-2(Ser731) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
FCM
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IRS-2 around the phosphorylation site of Ser731 |
| Modification Site | Ser731 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8660 |
| Synonyms | Insulin Receptor Substrate 2; IRS 2; IRS2. |
| Background | The family of insulin receptor substrates (IRSs) has been reported to play important roles for signal transduction of various hormones. Four members of the IRS family have been described. Each IRS is believed to have different functions; however, the distinct physiological roles of each IRS are unclear. Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. |
| Application Dilution | |
| FCM | 1:100 |
| IF(ICC) | 1:100 |
Frequently asked questions
Which applications is phospho-IRS-2(Ser731) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5397R-BF405) validated for, and at what dilution?
bs-5397R-BF405 is validated for FCM (1:100); IF(ICC) (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IRS-2(Ser731) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5397R-BF405) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Horse.
How should bs-5397R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IRS-2(Ser731) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-5397R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human IRS-2 around the phosphorylation site of Ser731
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-5397R-BF405)?
AbBy Fluor-405 on bs-5397R-BF405: excitation / emission 401nm/421nm.