Phospho-c-Kit (Ser741) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5400R-Cy3 |
| Product Name | Phospho-c-Kit (Ser741) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human c-Kit around the phosphorylation site of Ser741 |
| Modification Site | Ser741 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3815 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | c-Kit phospho S741; c-Kit phospho Ser741; p-c-Kit Ser 741; C Kit; c-Kit; CD 117; CD117; CD117 antigen; KIT; KIT_HUMAN; Mast/stem cell growth factor receptor; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto oncogene c Kit; Proto oncogene tyrosine protein kinase Kit; Proto-oncogene c-Kit; SCF Receptor; SCFR; soluble KIT variant 1; Stem cell factor receptor; tyrosine protein kinase Kit; Tyrosine-protein kinase Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog. |
| Background | c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-c-Kit (Ser741) Polyclonal Antibody, Cy3 Conjugated (bs-5400R-Cy3) validated for, and at what dilution?
bs-5400R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-c-Kit (Ser741) Polyclonal Antibody, Cy3 Conjugated (bs-5400R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-5400R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-c-Kit (Ser741) Polyclonal Antibody, Cy3 Conjugated (bs-5400R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human c-Kit around the phosphorylation site of Ser741
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5400R-Cy3)?
Cy3 on bs-5400R-Cy3: excitation / emission 512,550nm/570,615nm.