phospho-KCNC1 (Ser503) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5403R-Cy3 |
| Product Name | phospho-KCNC1 (Ser503) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human KCNC1 around the phosphorylation site of Ser503 |
| Modification Site | Ser503 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3746 |
| Subcellular location | Cell membrane |
| Synonyms | C230009H10Rik; FLJ41162; FLJ42249; FLJ43491; Kcr2 1; KShIIIB; Kv3.1; Kv4; MGC129855; NGK2; Potassium voltage-gated channel subfamily C member 1; Shaw; Voltage gated potassium channel; KCNC1_RAT; Voltage gated potassium channel subunit Kv3.1; Voltage-gated potassium channel subunit Kv3.1; KCNC1_RAT. |
| Background | KCNC1 mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. It forms a heteromultimer with KCNG3, KCNG4 and KCNV2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-KCNC1 (Ser503) Polyclonal Antibody, Cy3 Conjugated (bs-5403R-Cy3) validated for, and at what dilution?
bs-5403R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-KCNC1 (Ser503) Polyclonal Antibody, Cy3 Conjugated (bs-5403R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-5403R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-KCNC1 (Ser503) Polyclonal Antibody, Cy3 Conjugated (bs-5403R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human KCNC1 around the phosphorylation site of Ser503
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5403R-Cy3)?
Cy3 on bs-5403R-Cy3: excitation / emission 512,550nm/570,615nm.