Phospho-LRP6 (Thr1479) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5408R-Biotin-100uL |
| Product Name | Phospho-LRP6 (Thr1479) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human LRP6 around the phosphorylation site of Thr1479 |
| Modification Site | Thr1479 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 4040 |
| Swiss Prot | O75581 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | LRP6 Thr1479; LRP6 T1479; p-LRP6 Thr1479; p-LRP6 T1479; ADCAD2; C030016K15Rik; Cd; FLJ90062; FLJ90421; Low density lipoprotein receptor related protein 6; LRP6_HUMAN; Low-density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6 precursor; LRP-6. |
| Background | This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Phospho-LRP6 (Thr1479) Polyclonal Antibody, Biotin Conjugated (bs-5408R-Biotin) validated for, and at what dilution?
bs-5408R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-LRP6 (Thr1479) Polyclonal Antibody, Biotin Conjugated (bs-5408R-Biotin) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit, GuineaPig.
How should bs-5408R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-LRP6 (Thr1479) Polyclonal Antibody, Biotin Conjugated (bs-5408R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human LRP6 around the phosphorylation site of Thr1479