phospho-ASK1 (Ser1033) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5437R-BF488 |
| Product Name | phospho-ASK1 (Ser1033) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Ser1033 |
| Modification Site | Ser1033 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4217 |
| Subcellular location | Cytoplasm |
| Synonyms | ASK1 phospho S1033; Mitogen-activated protein kinase kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Apoptosis signal-regulating kinase 1; ASK-1; MAP3K5; ASK1; MAPKKK5; MEKK5; M3K5_HUMAN. |
| Background | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ASK1 (Ser1033) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-5437R-BF488) validated for, and at what dilution?
bs-5437R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5437R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ASK1 (Ser1033) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-5437R-BF488)?
KLH conjugated synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Ser1033
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-5437R-BF488)?
AbBy Fluor-488 on bs-5437R-BF488: excitation / emission 499nm/519nm.