phospho-ILK-1(Thr173) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5443R-Cy7 |
| Product Name | phospho-ILK-1(Thr173) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ILK-1 around the phosphorylation site of Thr173 |
| Modification Site | Thr173 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3611 |
| Swiss Prot | Q13418 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | P59; ILK-1; ILK-2; p59ILK; HEL-S-28; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK; ILK1; ILK2 |
| Background | Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ILK-1(Thr173) Polyclonal Antibody, Cy7 Conjugated (bs-5443R-Cy7) validated for, and at what dilution?
bs-5443R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ILK-1(Thr173) Polyclonal Antibody, Cy7 Conjugated (bs-5443R-Cy7) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-5443R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ILK-1(Thr173) Polyclonal Antibody, Cy7 Conjugated (bs-5443R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human ILK-1 around the phosphorylation site of Thr173
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5443R-Cy7)?
Cy7 on bs-5443R-Cy7: excitation / emission 743nm/767nm.