phospho-IGF1R (Tyr980) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5447R-APC |
| Product Name | phospho-IGF1R (Tyr980) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr980 |
| Modification Site | Tyr980 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3480 |
| Swiss Prot | P08069 |
| Subcellular location | Cell membrane |
| Synonyms | IGFR; CD221; IGFIR; JTK13; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; IGF1R |
| Background | When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IGF1R (Tyr980) Polyclonal Antibody, APC Conjugated (bs-5447R-APC) validated for, and at what dilution?
bs-5447R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IGF1R (Tyr980) Polyclonal Antibody, APC Conjugated (bs-5447R-APC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-5447R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IGF1R (Tyr980) Polyclonal Antibody, APC Conjugated (bs-5447R-APC)?
KLH conjugated synthetic phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr980
What are the excitation and emission wavelengths of the APC conjugate (bs-5447R-APC)?
APC on bs-5447R-APC: excitation / emission 650nm/660nm.