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phospho-ITGB4 (Tyr1494) Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-5455R-Biotin
Product Name phospho-ITGB4 (Tyr1494) Polyclonal Antibody, Biotin Conjugated
Applications
ELISA IHC-P IHC-F
Reactivity Rat
Predicted Reactivity Mouse, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from mouse ITGB4 around the phosphorylation site of Tyr1494
Modification Site Tyr1494
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 192897
Swiss Prot A2A863
Subcellular location Cell membrane
Synonyms CD104; AA407042; C230078O20; Integrin beta-4; Itgb4
Background Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility (By similarity).
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is phospho-ITGB4 (Tyr1494) Polyclonal Antibody, Biotin Conjugated (bs-5455R-Biotin) validated for, and at what dilution?

bs-5455R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-ITGB4 (Tyr1494) Polyclonal Antibody, Biotin Conjugated (bs-5455R-Biotin) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Rabbit.

How should bs-5455R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-ITGB4 (Tyr1494) Polyclonal Antibody, Biotin Conjugated (bs-5455R-Biotin)?

KLH conjugated synthetic phosphopeptide derived from mouse ITGB4 around the phosphorylation site of Tyr1494