phospho-IFNAR1 (Ser535+Ser539) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5457R-BF647 |
| Product Name | phospho-IFNAR1 (Ser535+Ser539) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IFNAR1 around the phosphorylation site of Ser535+Ser538 |
| Modification Site | Ser535+Ser538 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3454 |
| Swiss Prot | P17181 |
| Subcellular location | Cell membrane |
| Synonyms | AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC; Interferon alpha/beta receptor 1; IFN-R-1; IFN-alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1; IFNAR1 |
| Background | Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. Can also transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IFNAR1 (Ser535+Ser539) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-5457R-BF647) validated for, and at what dilution?
bs-5457R-BF647 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IFNAR1 (Ser535+Ser539) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-5457R-BF647) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse, GuineaPig.
How should bs-5457R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IFNAR1 (Ser535+Ser539) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-5457R-BF647)?
KLH conjugated synthetic phosphopeptide derived from human IFNAR1 around the phosphorylation site of Ser535+Ser538
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-5457R-BF647)?
AbBy Fluor-647 on bs-5457R-BF647: excitation / emission 650nm/665nm.