phospho-JunB (Ser259) Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5464R-Biotin |
| Product Name | phospho-JunB (Ser259) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Guinea Pig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human JunB around the phosphorylation site of Ser259 [PV(p-S)PI] |
| Modification Site | Ser259 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 3726 |
| Subcellular location | Nucleus |
| Synonyms | Activator protein 1; AP 1; AP1; Jun B; Jun B proto oncogene; Jun B protooncogene; JunB proto oncogene; JunB protoncogene 9; JunB protooncogene; Transcription factor jun B; Transcription factor junB. |
| Background | The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-JunB (Ser259) Polyclonal Antibody, Biotin Conjugated (bs-5464R-Biotin) validated for, and at what dilution?
bs-5464R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5464R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-JunB (Ser259) Polyclonal Antibody, Biotin Conjugated (bs-5464R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human JunB around the phosphorylation site of Ser259 [PV(p-S)PI]