phospho-gamma Catenin (Tyr550) Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5467R |
| Product Name | phospho-gamma Catenin (Tyr550) Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human JUP around the phosphorylation site of Tyr550 |
| Modification Site | Tyr550 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3728 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | gamma Catenin phospho Y550; p-gamma Catenin phospho Y550; catenin cadherin-associated protein gamma 80kD; Catenin gamma; CTNNG; Desmoplakin 3; Desmoplakin III; Desmoplakin3; DesmoplakinIII; DP 3; DP III; DP3; DPIII; gamma catenin; Junction plakoglobin; JUP; PDGB; PKGB; PLAKOGLOBIN. |
| Background | Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-gamma Catenin (Tyr550) Polyclonal Antibody (bs-5467R) validated for, and at what dilution?
bs-5467R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5467R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-gamma Catenin (Tyr550) Polyclonal Antibody (bs-5467R)?
KLH conjugated synthetic phosphopeptide derived from human JUP around the phosphorylation site of Tyr550