phospho-gamma Catenin (Tyr550) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5467R-Cy5 |
| Product Name | phospho-gamma Catenin (Tyr550) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human JUP around the phosphorylation site of Tyr550 |
| Modification Site | Tyr550 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3728 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | gamma Catenin phospho Y550; p-gamma Catenin phospho Y550; catenin cadherin-associated protein gamma 80kD; Catenin gamma; CTNNG; Desmoplakin 3; Desmoplakin III; Desmoplakin3; DesmoplakinIII; DP 3; DP III; DP3; DPIII; gamma catenin; Junction plakoglobin; JUP; PDGB; PKGB; PLAKOGLOBIN. |
| Background | Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-gamma Catenin (Tyr550) Polyclonal Antibody, Cy5 Conjugated (bs-5467R-Cy5) validated for, and at what dilution?
bs-5467R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5467R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-gamma Catenin (Tyr550) Polyclonal Antibody, Cy5 Conjugated (bs-5467R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human JUP around the phosphorylation site of Tyr550
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5467R-Cy5)?
Cy5 on bs-5467R-Cy5: excitation / emission 625,650nm/670nm.