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phospho-gamma Catenin (Tyr550) Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-5467R-FITC
Product Name phospho-gamma Catenin (Tyr550) Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human JUP around the phosphorylation site of Tyr550
Modification Site Tyr550
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3728
Subcellular location Cytoplasm, Cell membrane
Synonyms gamma Catenin phospho Y550; p-gamma Catenin phospho Y550; catenin cadherin-associated protein gamma 80kD; Catenin gamma; CTNNG; Desmoplakin 3; Desmoplakin III; Desmoplakin3; DesmoplakinIII; DP 3; DP III; DP3; DPIII; gamma catenin; Junction plakoglobin; JUP; PDGB; PKGB; PLAKOGLOBIN.
Background Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-gamma Catenin (Tyr550) Polyclonal Antibody, FITC Conjugated (bs-5467R-FITC) validated for, and at what dilution?

bs-5467R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-5467R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-gamma Catenin (Tyr550) Polyclonal Antibody, FITC Conjugated (bs-5467R-FITC)?

KLH conjugated synthetic phosphopeptide derived from human JUP around the phosphorylation site of Tyr550

What are the excitation and emission wavelengths of the FITC conjugate (bs-5467R-FITC)?

FITC on bs-5467R-FITC: excitation / emission 494nm/518nm.