phospho-MYL6 (Tyr29+Ser30) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5478R-FITC |
| Product Name | phospho-MYL6 (Tyr29+Ser30) Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MYL6 around the phosphorylation site of Tyr29+Ser30 |
| Modification Site | Tyr29+Ser30 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4637 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ESMLC; LC 17; LC17; LC17 GI; LC17 NM; LC17A; LC17B; MLC 3; MLC-3; MLC1SM; MLC3; MLC3NM; MLC3SM; MYL 6; MYL6; MYL6_HUMAN; Myosin light chain 3; Myosin light chain 6 alkali smooth muscle and non muscle; Myosin light chain A3; Myosin light chain alkali 3; Myosin light polypeptide 6 alkali smooth muscle and non muscle; Myosin light polypeptide 6; Smooth muscle and non muscle myosin alkali light chain; Smooth muscle and nonmuscle myosin light chain alkali 6; 17 kDa myosin light chain. |
| Background | Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MYL6 (Tyr29+Ser30) Polyclonal Antibody, FITC Conjugated (bs-5478R-FITC) validated for, and at what dilution?
bs-5478R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MYL6 (Tyr29+Ser30) Polyclonal Antibody, FITC Conjugated (bs-5478R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Sheep, Pig, Chicken.
How should bs-5478R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MYL6 (Tyr29+Ser30) Polyclonal Antibody, FITC Conjugated (bs-5478R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human MYL6 around the phosphorylation site of Tyr29+Ser30
What are the excitation and emission wavelengths of the FITC conjugate (bs-5478R-FITC)?
FITC on bs-5478R-FITC: excitation / emission 494nm/518nm.