MEF2A(Thr319) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5485r-apc-cy7 |
| Product Name | MEF2A(Thr319) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Bovine |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MEF2A around the phosphorylation site of Thr319 |
| Immunogen Range | 300-340/507 |
| Modification Site | Thr319 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4205 |
| Swiss Prot | Q02078 |
| Subcellular location | Nucleus |
| Synonyms | mef2; ADCAD1; RSRFC4; RSRFC9; Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1; MEF2A |
| Background | Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is MEF2A(Thr319) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5485R-APC-Cy7) validated for, and at what dilution?
bs-5485R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MEF2A(Thr319) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5485R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat, Bovine.
How should bs-5485R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MEF2A(Thr319) Polyclonal Antibody, APC-Cy7 Conjugated (bs-5485R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human MEF2A around the phosphorylation site of Thr319 Immunogen range: 300-340/507.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-5485R-APC-Cy7)?
APC-Cy7 on bs-5485R-APC-Cy7: excitation / emission 650nm/780nm.