phospho-MAPK8 ( Thr183 + Tyr185) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5487R-BF680 |
| Product Name | phospho-MAPK8 ( Thr183 + Tyr185) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MAPK9 around the phosphorylation site of Thr183+Tyr185 |
| Modification Site | Thr183 + Tyr185 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5601 |
| Subcellular location | Nucleus |
| Synonyms | MAPK8/MAPK9phospho T183/Y185; Mapk9; C Jun kinase 2; C Jun N terminal kinase 2; JNK 2; JNK-2; JNK 55; JNK2 alpha; JNK2 beta; JNK2alpha; JNK2B; JNK2beta; JNK55; Jun kinase; MAP kinase 9; MAPK 9; Mitogen activated protein kinase 9; P54 alpha; P54aSAPK; PRKM9; SAPK alpha; SAPK1a; SAPKa; Stress activated protein kinase JNK2. |
| Background | phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MAPK8 ( Thr183 + Tyr185) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-5487R-BF680) validated for, and at what dilution?
bs-5487R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MAPK8 ( Thr183 + Tyr185) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-5487R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-5487R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MAPK8 ( Thr183 + Tyr185) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-5487R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human MAPK9 around the phosphorylation site of Thr183+Tyr185
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-5487R-BF680)?
AbBy Fluor-680 on bs-5487R-BF680: excitation / emission 679nm/702nm.