phospho-MAP4K4 (Ser629) Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5491R-RBITC-100uL |
| Product Name | phospho-MAP4K4 (Ser629) Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MAP4K4 around the phosphorylation site of Ser629 |
| Modification Site | Ser629 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9448 |
| Subcellular location | Cytoplasm |
| Synonyms | MAP4K4phospho S629; Hepatocyte progenitor kinase like/germinal center kinase like kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; KIAA0687; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK. |
| Background | MAP4K4 (Mitogen-activated protein kinase kinase kinase kinase 4) belongs to the serine/threonine protein kinase family and specifically activates MAPK8/JNK. It may play a role in the response to environmental stress and is thought to function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF alpha signaling pathway. It interacts with the SH3 domain of the adapter protein Nck. MAP4K4-dependent signaling inhibits PPARG responsive gene expression, adipogenesis, and insulin stimulated glucose transport. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MAP4K4 (Ser629) Polyclonal Antibody, RBITC Conjugated (bs-5491R-RBITC) validated for, and at what dilution?
bs-5491R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5491R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MAP4K4 (Ser629) Polyclonal Antibody, RBITC Conjugated (bs-5491R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human MAP4K4 around the phosphorylation site of Ser629
What are the excitation and emission wavelengths of the RBITC conjugate (bs-5491R-RBITC)?
RBITC on bs-5491R-RBITC: excitation / emission 540nm/625nm.