phospho-MEF2D (Ser180) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5501R-Cy3 |
| Product Name | phospho-MEF2D (Ser180) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MEF2D around the phosphorylation site of Ser180 |
| Modification Site | Ser180 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4209 |
| Subcellular location | Nucleus |
| Synonyms | MEF2Dphospho S180; MADS box transcription factor 2 polypeptide D; Mef2d; MEF2D_HUMAN; y myocyte enhancer factor 2D; Myocyte specic enhancer factor 2D; myocyte-specic enhancer factor 2D. |
| Background | Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MEF2D (Ser180) Polyclonal Antibody, Cy3 Conjugated (bs-5501R-Cy3) validated for, and at what dilution?
bs-5501R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MEF2D (Ser180) Polyclonal Antibody, Cy3 Conjugated (bs-5501R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5501R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MEF2D (Ser180) Polyclonal Antibody, Cy3 Conjugated (bs-5501R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human MEF2D around the phosphorylation site of Ser180
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5501R-Cy3)?
Cy3 on bs-5501R-Cy3: excitation / emission 512,550nm/570,615nm.