phospho-MEF2D (Ser444) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5502R-Cy7 |
| Product Name | phospho-MEF2D (Ser444) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MEF2D around the phosphorylation site of Ser444 |
| Modification Site | Ser444 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4209 |
| Subcellular location | Nucleus |
| Synonyms | MEF2Dphospho S444; DKFZp686I1536; MADS box transcription factor 2 polypeptide D; Mef2d; MEF2D_HUMAN; myocyte enhancer factor 2D; Myocyte specic enhancer factor 2, polypeptide D; Myocyte specic enhancer factor 2D; myocyte-specic enhancer factor 2D. |
| Background | MEF2D is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MEF2D (Ser444) Polyclonal Antibody, Cy7 Conjugated (bs-5502R-Cy7) validated for, and at what dilution?
bs-5502R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MEF2D (Ser444) Polyclonal Antibody, Cy7 Conjugated (bs-5502R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.
How should bs-5502R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MEF2D (Ser444) Polyclonal Antibody, Cy7 Conjugated (bs-5502R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human MEF2D around the phosphorylation site of Ser444
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5502R-Cy7)?
Cy7 on bs-5502R-Cy7: excitation / emission 743nm/767nm.