Caspase-3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-55032r-percp-cy55-100ul |
| Product Name | Caspase-3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2). |
| Immunogen Range | 55-160/277 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 836 |
| Swiss Prot | P42574 |
| Subcellular location | Cytoplasm |
| Synonyms | CPP32;CPP32B;SCA-1;Active Caspase 3;CASP3;active Caspase-3;Caspase 3;Caspase-3 p12;caspase-3 |
| Background | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is Caspase-3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-55032R-PERCP-CY5-5) validated for, and at what dilution?
BS-55032R-PERCP-CY5-5 is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-55032R-PERCP-CY5-5) react with?
Tested reactivity: Human.
How should BS-55032R-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase-3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-55032R-PERCP-CY5-5)?
Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2). Immunogen range: 55-160/277.
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-55032R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-55032R-PERCP-CY5-5: excitation / emission 482nm/694nm.