CRKL Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: CRKL knockout (KO) HeLa cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: CRKL knockout (KO) HeLa cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: LO2 cell lysates; Lane 2: Jurkat cell lysates; Lane 3: SKOV3 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat heart cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: LO2 cell lysates; Lane 2: Jurkat cell lysates; Lane 3: SKOV3 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat heart cell lysates probed with CRKL Polyclonal Antibody, Unconjugated (bs-55046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55046R
Product Name	CRKL Polyclonal Antibody
Applications	WB, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1399
Swiss Prot	P46109
Subcellular location	Cytoplasm, Nucleus
Synonyms	Crk L; Crk like protein; Crkol; HGNC:2363; Oncogene CrkL; V crk avian sarcoma virus CT10 oncogene homolog like; V crk sarcoma virus CT10 oncogene homolog avian like; CRKL_HUMAN.
Background	This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Application Dilution
WB	1:300-5000
IF

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