CSPG4 Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: CSPG4 knockout (KO) HeLa cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: CSPG4 knockout (KO) HeLa cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: A375 cell lysates; Lane 2: Hela cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: A375 cell lysates; Lane 2: Hela cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55049R
Product Name	CSPG4 Polyclonal Antibody
Applications	WB, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1464
Swiss Prot	Q6UVK1
Subcellular location	Cytoplasm, Cell membrane
Synonyms	CSPG4;HMW-MAA;MCSP;MCSPG;MEL-CSPG;MSK16;NG2
Background	A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Application Dilution
WB	1:300-5000
IF

Powered by Bioz See more details on Bioz