Cytokeratin-8 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55051R |
| Product Name | Cytokeratin-8 Polyclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IF
IHC-P
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin-8 (NP_002264.1). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 3856 |
| Swiss Prot | P05787 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | KRT8;CARD2;CK-8;CK8;CYK8;K2C8;K8;KO;keratin 8 |
| Background | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Cytokeratin-8 Polyclonal Antibody (bs-55051R) validated for, and at what dilution?
bs-55051R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytokeratin-8 Polyclonal Antibody (bs-55051R) react with?
Tested reactivity: Human, Mouse.
How should bs-55051R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytokeratin-8 Polyclonal Antibody (bs-55051R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin-8 (NP_002264.1).