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DFFA Polyclonal Antibody

Lane 1: Human Jurkat cell lysates; Lane 2: Human H-460 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat Brain lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Jurkat cell lysates; Lane 2: Human H-460 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat Brain lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: DFFA knockout (KO) HeLa cell lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: DFFA knockout (KO) HeLa cell lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc

Applications

  • WB
  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-55054R
Product Name DFFA Polyclonal Antibody
Applications WB, IF(ICC), IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1676
Swiss Prot O00273
Subcellular location Cytoplasm, Cell membrane
Synonyms DFFA;DFF-45;DFF1;ICAD
Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200
IF

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