GSS Polyclonal Antibody

$Lane 1: DU145 cell lysates; Lane 2: K562 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Brain lysates; Lane 7: Rat Kidney lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: DU145 cell lysates; Lane 2: K562 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Brain lysates; Lane 7: Rat Kidney lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: 293T cell lysates; Lane 2: GSS knockout (KO) 293T cell lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: 293T cell lysates; Lane 2: GSS knockout (KO) 293T cell lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55091R
Product Name	GSS Polyclonal Antibody
Applications	WB IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2937
Swiss Prot	P48637
Subcellular location	Cytoplasm
Synonyms	GSS;GSHS;HEL-S-64p;HEL-S-88n
Background	Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400