HMGB1 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55099R-100uL |
| Product Name | HMGB1 Polyclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 3146 |
| Swiss Prot | P09429 |
| Subcellular location | Nucleus |
| Synonyms | HMG-1;HMG1;HMG3;SBP-1;HMGB1 |
| Background | This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is HMGB1 Polyclonal Antibody (bs-55099R) validated for, and at what dilution?
bs-55099R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HMGB1 Polyclonal Antibody (bs-55099R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-55099R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HMGB1 Polyclonal Antibody (bs-55099R)?
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).