IDE Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55107R |
| Product Name | IDE Polyclonal Antibody |
| Applications |
WB
IF(ICC)
IF
IHC-P
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 3416 |
| Swiss Prot | P14735 |
| Subcellular location | Cytoplasm, Cell membrane, Extracellular domain |
| Synonyms | IDE;INSULYSIN |
| Background | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is IDE Polyclonal Antibody (bs-55107R) validated for, and at what dilution?
bs-55107R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IDE Polyclonal Antibody (bs-55107R) react with?
Tested reactivity: Human, Mouse.
How should bs-55107R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IDE Polyclonal Antibody (bs-55107R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).