Phospho-NFKB1 (Thr931) Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5510R-PerCP-100uL |
| Product Name | Phospho-NFKB1 (Thr931) Polyclonal Antibody, PerCP Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human NF KappaB p105 around the phosphorylation site of Thr931 |
| Modification Site | Thr931 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4790 |
| Swiss Prot | P19838 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NFKB1(phospho T931); NFKB1(phospho Thr931); NF kappa B; NFKB 1; NFKB p105; NFKB p50; NFKB1; Nuclear factor kappa B DNA binding subunit; Nuclear factor NF kappa B p105 subunit; Nuclear factor NF kappa B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1; DKFZp686C01211; DNA binding factor KBF1; DNA binding factor KBF1 EBP1; EBP 1; EBP1; KBF1; MGC54151; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nfkb1; NF-kappaB; NF-kappaB1; NF-KB1; p105; p50; p50/p105; DKFZp686C01211; EBP-1; KBF1; MGC54151; Nf kappa b DNA binding subunit; NF-KAPPAB; NF-KAPPAB1; NFKB; NFKB SUBUNIT P105/P50; NFKB-p105; NFKB1; NUCLEAR FACTOR KAPPA B DNA BINDING SUBUNIT; p105 Nfkb;p50 NF-kappa B;p50NFKB. |
| Background | This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. SUBUNIT: Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-NFKB1 (Thr931) Polyclonal Antibody, PerCP Conjugated (bs-5510R-PerCP) validated for, and at what dilution?
bs-5510R-PerCP is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-NFKB1 (Thr931) Polyclonal Antibody, PerCP Conjugated (bs-5510R-PerCP) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Chicken.
How should bs-5510R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-NFKB1 (Thr931) Polyclonal Antibody, PerCP Conjugated (bs-5510R-PerCP)?
KLH conjugated Synthesised phosphopeptide derived from human NF KappaB p105 around the phosphorylation site of Thr931
What are the excitation and emission wavelengths of the PerCP conjugate (bs-5510R-PerCP)?
PerCP on bs-5510R-PerCP: excitation / emission 482nm/677nm.