MAP2K4 Polyclonal Antibody

$Lane 1: Hela cell lysates; Lane 2: MAP2K4 knockout (KO) HeLa cell lysates probed with MAP2K4 Polyclonal Antibody, Unconjugated (bs-55128R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Hela cell lysates; Lane 2: MAP2K4 knockout (KO) HeLa cell lysates probed with MAP2K4 Polyclonal Antibody, Unconjugated (bs-55128R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: SH-SY5Y cell lysates; Lane 2: BT-474 cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Mouse Thymus lysates; Lane 5: Mouse Liver lysates; Lane 6: Rat Liver lysates probed with SIRT4 Polyclonal\/ Monoclonal Antibody, Unconjugated (bs-55128R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: SH-SY5Y cell lysates; Lane 2: BT-474 cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Mouse Thymus lysates; Lane 5: Mouse Liver lysates; Lane 6: Rat Liver lysates probed with SIRT4 Polyclonal\/ Monoclonal Antibody, Unconjugated (bs-55128R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55128R
Product Name	MAP2K4 Polyclonal Antibody
Applications	WB IHC IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6416
Swiss Prot	P45985
Subcellular location	Nucleus
Synonyms	MAP2K4;JNKK;JNKK1;MAPKK4;MEK4;MKK4;PRKMK4;SAPKK-1;SAPKK1;SEK1;SERK1;SKK1
Background	This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Application Dilution
WB	1:300-5000
IHC
IF