MLKL Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-55131r-pe-cy5-100uL |
| Product Name | MLKL Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | Recombinant protein of mouse MLKL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 74568 |
| Swiss Prot | Q9D2Y4 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | hMLKL; 9130019I15Rik; MLKL_HUMAN; MLKL; MLKL_MOUSE; mixed lineage kinase domain like pseudokinase; mixed lineage kinase domain-like |
| Background | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is MLKL Polyclonal Antibody, PE-Cy5 Conjugated (bs-55131R-PE-Cy5) validated for, and at what dilution?
bs-55131R-PE-Cy5 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MLKL Polyclonal Antibody, PE-Cy5 Conjugated (bs-55131R-PE-Cy5) react with?
Tested reactivity: Human.
How should bs-55131R-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MLKL Polyclonal Antibody, PE-Cy5 Conjugated (bs-55131R-PE-Cy5)?
Recombinant protein of mouse MLKL
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-55131R-PE-Cy5)?
PE-Cy5 on bs-55131R-PE-Cy5: excitation / emission 488nm/670nm.