NCOA2 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55139R |
| Product Name | NCOA2 Polyclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 550-820 of human NCOA2 (NP_006531.1). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 10499 |
| Swiss Prot | Q15596 |
| Subcellular location | Nucleus |
| Synonyms | NCOA2;GRIP1;KAT13C;NCoA-2;SRC2;TIF2;bHLHe75 |
| Background | The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-F | 1:400-800 |
| IF | 1:100-500 |
| IHC-P | 1:100-500 |
Frequently asked questions
Which applications is NCOA2 Polyclonal Antibody (bs-55139R) validated for, and at what dilution?
bs-55139R is validated for WB (1:500-2000); IHC-F (1:400-800); IF (1:100-500); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NCOA2 Polyclonal Antibody (bs-55139R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-55139R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCOA2 Polyclonal Antibody (bs-55139R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 550-820 of human NCOA2 (NP_006531.1).