NDUFB4 Polyclonal Antibody

$L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Lane 1: U-87MG cell lysates; Lane 2: Hela cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Heart lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Liver lysates probed with NDUFB4 Polyclonal Antibody, Unconjugated (bs-55145R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: NDUFB4 knockout (KO) HeLa cell lysates probed with NDUFB4 Polyclonal Antibody, Unconjugated (bs-55145R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human MOLT4 cell lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human U-2 OS cell lysates probed with NDUFB4 Polyclonal Antibody, Unconjugated (bs-55145R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human MOLT4 cell lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human U-2 OS cell lysates probed with NDUFB4 Polyclonal Antibody, Unconjugated (bs-55145R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55145R
Product Name	NDUFB4 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	4710
Swiss Prot	O95168
Subcellular location	Cytoplasm
Synonyms	NDUFB4;B15;CI-B15
Background	This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.
Application Dilution
WB	1:300-5000
IHC-P	IFICC1:50-200
IHC-F	IF
IF