NDUFS3 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nNDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nNDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nNDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nNDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4\u00b0C, DAB staining.

Lane 1: MCF-7 cell lysates; Lane 2: SW620 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Kdiney lysates; Lane 5: Mouse Liver lysates; Lane 6: Mouse Heart lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Heart lysates probed with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: 293T cell lysates; Lane 2: NDUFS3 knockout (KO) 293T cell lysates probed with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55148R
Product Name	NDUFS3 Polyclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4722
Swiss Prot	O75489
Subcellular location	Cytoplasm, Cell membrane
Synonyms	NDUFS3;CI-30
Background	This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC

Applications

Reactivity

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