phospho-IKB alpha (Tyr42) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5514R-Cy7-100uL |
| Product Name | phospho-IKB alpha (Tyr42) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IKB alpha around the phosphorylation site of Tyr42 |
| Modification Site | Tyr42 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4792 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NFKBIATyr42; NFKBIAphospho Y42; IKB-alphaTyr42; IKB alpha; Inhibitor of KB alpha; I kappa B alpha; IKappaBalpha; IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha. |
| Background | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IKB alpha (Tyr42) Polyclonal Antibody, Cy7 Conjugated (bs-5514R-Cy7) validated for, and at what dilution?
bs-5514R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IKB alpha (Tyr42) Polyclonal Antibody, Cy7 Conjugated (bs-5514R-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Rabbit.
How should bs-5514R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IKB alpha (Tyr42) Polyclonal Antibody, Cy7 Conjugated (bs-5514R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human IKB alpha around the phosphorylation site of Tyr42
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5514R-Cy7)?
Cy7 on bs-5514R-Cy7: excitation / emission 743nm/767nm.