P21 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-55160R
Product Name P21 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F
Specificity KO-Validated
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human P21 (NP_001207707.1).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1026
Swiss Prot P38936
Subcellular location Cytoplasm, Nucleus
Synonyms CAP20;CDKN1;CIP1;MDA-6;P21;SDI1;WAF1;p21CIP1;CDKN1A
Background This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Application Dilution
WB 1:300-1000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500